human monocytic leukemia cell line (ATCC)

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ATCC human monocytic leukemia cell line
Human Monocytic Leukemia Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 20139 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+leukemia+monocytic+thp+1+cells/THP-1/pmc13126472-368-12-25
Average 99 stars, based on 20139 article reviews

human monocytic leukemia cell line - by Bioz Stars, 2026-09

99/100 stars

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CRISPR:

Article Title: REDD1 expression in podocytes facilitates renal inflammation and pyroptosis in streptozotocin-induced diabetic nephropathy
Article Snippet: .. Human leukemia monocytic THP-1 cells (ATCC TIB-202) were differentiated into macrophages using 50 ng/mL phorbol 12-myristate-13-acetate (Cayman Chemicals, Ann Arbor, MI, USA) for 48 h. CRISPR/Cas9 genome editing was used to generate a stable CIHP-1 REDD1 knockout (REDD1 KO) cell line [ ]. .. Plasmids including a pCMV5 vector (EV), HA-tagged pCMV-HA-REDD1, or pBabe-GFP-IκB alpha-mut (IκBα super repressor; Addgene plasmid # 15264) were transiently transfected using Jet PRIME (Polyplus transfection, New York, NY, USA).

Article Title: REDD1 expression in podocytes facilitates renal inflammation and pyroptosis in streptozotocin-induced diabetic nephropathy.
Article Snippet: .. Human leukemia monocytic THP-1 cells (ATCC TIB-202) were differentiated into macrophages using 50 ng/mL phorbol 12-myristate-13acetate (Cayman Chemicals, Ann Arbor, MI, USA) for 48 h. CRISPR/Cas9 genome editing was used to generate a stable CIHP-1 REDD1 knockout (REDD1 KO) cell line [16]. .. Plasmids including a pCMV5 vector (EV), HAtagged pCMV-HA-REDD1, or pBabe-GFP-IκB alpha-mut (IκBα super repressor; Addgene plasmid # 15264) were transiently transfected using Jet PRIME (Polyplus transfection, New York, NY, USA).

Knock-Out:

Cell Culture:

Article Title: Targeting phenol-soluble modulin α3-driven M1 macrophage polarization and necroptosis mitigates MRSA infection in mice.
Article Snippet: .. Human leukemia monocytic THP-1 cells (TIB-202, ATCC) were cultured in RPMI-1640 medium supplemented with 10% FBS, 1% penicillinstreptomycin, and 50 μM β-mercaptoethanol at 37°C with 5% CO2. .. Differentiation of THP-1 monocytes to macrophage was induced by treatment with 100 ng/mL PMA for 24 h. MLKL knockout THP-1 cells (YKO-HT0390, Ubigene) were cultured and confirmed by Western blot analysis (Supplementary Figure 16).

Article Title: Intercellular Horizontal Transfer of TXNDC5 mRNA via Extracellular Vesicles Contributes to Tumor‐Associated Macrophage‐Mediated Prostate Cancer Metastasis
Article Snippet: .. Human leukemia monocytic THP‐1 cells (TIB‐202, ATCC, RRID: CVCL_0006) and human PCa cell lines PC3 (CRL‐1435, ATCC, RRID: CVCL_0035) were cultured in RPMI‐1640 medium (72400047, Gibco) containing 10% fetal bovine serum (FBS) (BS1612‐109, Bioexplorer) and 1% penicillin‐streptomycin solution (100X, BL505A, Biosharp). .. For THP‐1, medium was additionally supplied with β‐mercaptoethanol (0.05 m m , M8211, Solarbio).

Article Title: Intercellular Horizontal Transfer of TXNDC5 mRNA via Extracellular Vesicles Contributes to Tumor-Associated Macrophage-Mediated Prostate Cancer Metastasis.
Article Snippet: .. Human leukemia monocytic THP-1 cells (TIB-202, ATCC, RRID: CVCL_0006) and human PCa cell lines PC3 (CRL1435, ATCC, RRID: CVCL_0035) were cultured in RPMI-1640 medium (72400047, Gibco) containing 10% fetal bovine serum (FBS) (BS1612-109, Bioexplorer) and 1% penicillin-streptomycin solution (100X, BL505A, Biosharp). .. For THP-1, medium was additionally supplied with β-mercaptoethanol (0.05 m m , M8211, Solarbio).

Activity Assay:

Article Title: In vitro anti-neuroinflammatory and antioxidant activities of mushroom supercritical CO2 extracts
Article Snippet: .. Human leukemia monocytic (THP-1) cells (ATCC, Manassa, VA, USA) differentiated into macrophages using 100 ng/mL phorbol 12-myristate 13-acetate (PMA) were used to determine the anti-inflammatory activity following the protocol detailed in Villalva et al. (2018). ..

Review